When PNMT crystals are grown in non-reducing solutions, two disulfide bonds form between cysteines 48 and 139 on opposite chains.
2.
A mixture of haemoglobin S ( Hb S ) in a reducing solution ( such as sodium dithionite ) gives a turbid appearance, whereas normal Hb gives a clear solution.
3.
It was demonstrated that when the reduced solution of trunculus dye is exposed to sunlight, the UV-rays from the sun act to break the bromine bonds such that when oxidation occurs following the removal of the dyed fabric from the solution, pure indigo bonds to the wool, while the bromine atoms are left in the vat.
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